Impact of Diuretics on Metabolic Activity of Urogenital Tract Microbiota in Women
Amar Balihodžić1, Nadira Ibrišimović Mehmedinović2, Suada Tinjić3, Semira Galijašević4, Kamelija Madacki-Todorović5, Izet Eminović6, Lejla Hasanbegović7, Mirza Ibrišimović8

1Amar Balihodžić*, Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina.
2Nadira Ibrišimović Mehmedinović, Department of Chemistry, Faculty of Natural Science and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina.
3Suada Tinjić, Gynecology Polyclinic “Korak do života”, Tuzla, Bosnia and Herzegovina. 
4Semira Galijašević, Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina.
5Kamelija Madacki-Todorović, Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina.
6Izet Eminović, Faculty of Natural Science and Mathematics, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
7Lejla Hasanbegović, Biochemical-Immunological-Hemathological “Medical Laboratory” Ilidža, Sarajevo, Bosnia and Herzegovina.
8Mirza Ibrišimović, Gynecology Polyclinic “Korak do života”, Tuzla, Bosnia and Herzegovina and Sarajevo Medical School, University Sarajevo School of Science and Technology.
Manuscript received on July 18, 2021. | Revised Manuscript received on September 1, 2021. | Manuscript published on October 30, 2021. | PP: 27-33 | Volume-11 Issue-1, October 2021. | Retrieval Number: 100.1/ijeat.F29880810621 | DOI: 10.35940/ijeat.A31171011121
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Abstract: Limited knowledge exists about the effects of commonly used diuretic medications on the human normal flora. Thus, we investigated potential stimulatory effects of diuretic drug furosemide on urogenital tract microbiota in women. Three strains of E. coli and C. albicans with different biofilm forming capacities were obtained from female patients diagnosed with urinary tract infections. All tested strains were treated with two different concentrations of furosemide drug, in comparison to non-treated strains as the negative control. At specific time intervals, samples were obtained from growing culture and analyzed for their proliferation rate, aspartyl proteinase excretion and biofilm formation ability. E. coli and C. albicans strains significantly increased their aspartyl proteinase excretion under furosemide treatment. This effect was frequently observed after 16 hours of incubation at 37oC. This drug has also increased the biofilm forming capacities of E. coli and C. albicans strains. Interestingly, both E. coli and C. albicans non-biofilm former strains, gained the capacity of biofilm formation when treated with furosemide at certain concentrations. E. coli control became a weak biofilm former after 48 hours of incubation, while non-biofilm former C. albicans strain became a weak biofilm former in dose-dependent fashion, after 48 hours incubation with furosemide in concentration of 0.1 mg/mL, and after 16 hours of incubation with furosemide in concentration of 0.5 mg/mL. Loop.
Keywords: Specific time intervals, samples were obtained from growing culture and analyzed for their proliferation rate, aspartyl proteinase excretion and biofilm formation ability.