Application of Plackett Burman Design for Antioxidant Extraction from Actinodaphne Sesquipedalis leaves
Siti Fatihah Balqis Binti Jamaludin1, Siti Salwa Binti Abd Gani2,Uswatun Hasanah Binti Zaidan3, Paiman Bin Bawon4
1Siti Fatihah Balqis Binti Jamaludin, Halal Products Research Institute, University Putra Malaysia, Serdang, Malaysia.
2Siti Salwa Binti Abd Gani, Department of Agriculture Technology, Faculty of Agriculture, University Putra Malaysia, Serdang, Malaysia.
3Uswatun Hasanah Binti Zaidin, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang, Malaysia.
4Paiman Bin Bawon, Department of Forest Production, Faculty of Forestry, University Putra Malaysia, Serdang, Malaysia.
Manuscript received on 27 September 2019 | Revised Manuscript received on 09 November 2019 | Manuscript Published on 22 November 2019 | PP: 48-51 | Volume-8 Issue-6S3 September 2019 | Retrieval Number: F10080986S319/19©BEIESP | DOI: 10.35940/ijeat.F1008.0986S319
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Abstract: Actinodaphne sesquipedalis Hook. F. Var. Glabra (Kochummen), also known as “Medang payung” by the Malay people, belongs to the Lauraceae family. In this study, a Plackett-Burman design was used to evaluate the significant extraction parameters in achieving maximum DPPH radical scavenging activity from ethanol leaves extract of A. sesquipedalis. Microwave-assisted extraction technique was employed using aqueous ethanol. The independent parameters were microwave power level (30–60 W), feed-to-solvent ratio (1:30 g/ml), irradiation time (30-90 s) and ethanol concentration (20–80%). Amongst the examined parameters, ethanol concentration, microwave power level, and irradiation/extraction time were significant, whereas, feed-to-solvent ratio was insignificant. Therefore, antioxidants from the ethanolic extraction leaves of A. sesquipedalis using microwave technique are significantly affected by ethanol concentration, irradiation time and microwave power.
Keywords: Actinodaphne Sesquipedalis, DPPH Radical Scavenging Activity, Microwave, Plackett-Burman Design.
Scope of the Article: Bio-Science and Bio-Technology