Measurement of Fluorescence Lifetimes using Minimum Norm and Multiparameter Deconvolution
Abdussamad U. Jibia
Dr. Abdussamad U. Jibia, Department of Electrical Engineering, Bayero University Kano, Nigeria.
Manuscript received on May 15, 2012. | Revised Manuscript received on June 07, 2012. | Manuscript published on June 30, 2012. | PP: 519-525 | Volume-1 Issue-5, June 2012. | Retrieval Number: E0462061512/2012©BEIESP

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Abstract: A new method of fluorescence decay analysis is presented. The method relies on the classical Gardner transform to convert the fluorescence decay data into a convolution model which is deconvolved using multiparameter deconvolution technique. Minimum norm eigenvector method is then used to further model the resulting complex exponentials to obtain better estimates of fluorescence decay rates and number of components. Simulation results indicate that the SNR detection threshold is very low compared with several other methods. Fluorescence decay data is finally postprocessed using the proposed approach. The results are motivating. 
Keywords: Fluorescence, Gardner transform, multiexponential, multiparameter deconvolution, minimum norm.