Pilot Scale Production of AZOTOBACTER BIOFERTILIZER and Its Effect on the Growth Parameters of OCIMUM Sanctum
M.Shanmuga Priya1, Reyaz Farooq2, Divyashree K3, A.K Satheesh Babu4, M.Lakshmi Prabha5, Prasad M.P6
1Dr. M Shanmuga Priya, Department of Biotechnology, MVJ College of Engineering, Bangalore, India.
2Reyaz Farooq, Department of Biotechnology, MVJ College of Engineering, Bangalore, India.
3Divyashree K, Department of Biotechnology, MVJ College of Engineering, Bangalore, India.
4Dr. A.K Satheesh Babu, Department of Biotechnology, MVJ College of Engineering, Bangalore, India.
5Dr. M. Lakshmi Prabha, Department of Biotechnology, Karunya University Coimbatore, India.
6Dr. Prasad M.P, Sangenomics Research Laboratory, Bangalore, India.
Manuscript received on March 02, 2013. | Revised Manuscript received on April 14, 2013. | Manuscript published on April 30, 2013. | PP: 534-537 | Volume-2, Issue-4, April 2013. | Retrieval Number: D1518042413/2013©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Azotobacters are predominant in the rhizosphere of plants and they help in phosphate solubilisation. They belong to the family Azotobacteraceae and they are used as broad spectrum biofertilizers. In the present study four samples were collected from the rhizosphere regions of the plants namely Solanum lycopersicum (tomato), Zingiber officinale (ginger), Solanum melongena (Brinjal) and Allium sativum (Garlic). The collected samples were isolated and identified as isolates 1, 2, 3 and 4. Biochemical tests such as motility test, catalase test, oxidase test, starch hydrolysis, litmus milk and phosphate solubilisation were performed. The effect of culture parameters such as Incubation period, pH, Temperature and carbon source at different concentrations were carried out. Mass production of Azotobacter was carried out using batch fermenter. Further pot experiments were conducted to determine the root length, shoot length and number of leaves in Ocimum sanctum on 15th , 30th and 45th day. The results showed an increase in root length, shoot length and number of leaves in biofertilizer treated plant when compared with the control and inorganic fertilizer.
Keywords: Azotobacter, Biofertlizer, Ocimum sanctum and Rhizosphere.