Effect of Temperature on 60nm and 100nm Nano sphere Size Standard using Dynamic Light Scattering
Nor Dyana Zakaria1, Izzati Zahidah Abdul Karim2, Azimah Ahmad3, Md. Sohrab Hossain4, Venugopal Balakrishnan5

1Nor Dyana Zakaria, Institute for Research in Molecular Medicine, University Sains Malaysia, Penang, Malaysia.
2Izzati Zahidah Adbul Karim, Institute for Research in Molecular Medicine, University Sains Malaysia, Penang, Malaysia.
3Azimah Ahmad, Institute for Research in Molecular Medicine, University Sains Malaysia, Penang, Malaysia.
4Md. Sohrab Hossain, Environmental Technology Division, School of Institute for Research in Molecular Medicine, University Sains Malaysia, Penang, Malaysia.
5Venugopal Balakrishnan*, Institute for Research in Molecular Medicine, University Sains Malaysia, Penang, Malaysia.
Manuscript received on November 23, 2019. | Revised Manuscript received on December 15, 2019. | Manuscript published on December 30, 2019. | PP: 3284-3286 | Volume-9 Issue-2, December, 2019. | Retrieval Number:  B3371129219/2019©BEIESP | DOI: 10.35940/ijeat.B3371.129219
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Abstract: It is difficult to use kinetic motion as a measurement tool without temperature affecting the results. In this study, the size of a known nanosphere was used to monitor the effect of temperature towards particle size determination using dynamic light scattering principles. Temperature deviations are always picked up by size measurement using the principle of Brownian motion. The particle size of 60nm and 100nm polystyrene latex nanoparticles in 10mM NaCl solution was measured at four different temperatures set points of 20, 25, 30, 35 and 40°C using dynamic light scattering mechanism. As a result, the size of polystyrene latex nanoparticles was increased with the increases of the temperature. Therefore, for particle size analysis using dynamic light scattering mechanism the temperature of the test must be maintained at 25°C in order to obtain accurate measurement.
Keywords: Particles size, Temperature, Dynamic light scattering.