Selection of Promising Lactobacilli Antagonistic to Campylobacter Jejuni
Svetlana Makavchik1, Larisa Karpenko2, Yuri Kuznetsov3, Ilya Nikonov4, Alesya Bakhta5
1Svetlana Makavchik*, St. Petersburg State Academy of Veterinary Medicine, Chernigovskaya, Saint Petersburg, Russia.
2Larisa Karpenko, St. Petersburg State Academy of Veterinary Medicine, Chernigovskaya, Saint Petersburg, Russia.
3Yuri Kuznetsov, St. Petersburg State Academy of Veterinary Medicine, Chernigovskaya, Saint Petersburg, Russia.
4Ilya Nikonov, Federal State Budget Scientific Institution Federal Scientific Center “All-Russian Research and Technological Poultry Institute” of Russian Academy of Sciences, Pticegradskaya, Sergiev Posad, Moscow region, Russia.
5Alesya Bakhta, St. Petersburg State Academy of Veterinary Medicine, Chernigovskaya, Saint Petersburg, Russia.
Manuscript received on September 21, 2019. | Revised Manuscript received on October 15, 2019. | Manuscript published on October 30, 2019. | PP: 3983-3986 | Volume-9 Issue-1, October 2019 | Retrieval Number: A9965109119/2019©BEIESP | DOI: 10.35940/ijeat.A9965.109119
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: This article reflects the results of a study on the selection of promising lactobacilli antagonistic to Campilobacter jejuni, a strain that is the most common and more pathogenic for humans, carried out as part of a project to scientifically substantiate the use of new technologies in poultry feeding using special probiotic strains that increase productivity and obtaining poultry products of improved quality with the properties of functional food products. During the study, strains of lactic acid bacteria were obtained. The cultivation of strains of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus plantarum, Lactobacillus plantarum was carried out on liquid and agarized nutrient media MRS at 370С for 24 hours. In vitro antagonistic studies were performed using the two-way antagonistic method on a wide range of indicator crops. Since there is evidence of a specific mechanism for the manifestation of the antagonistic activity of lactobacilli to gram-negative and gram-positive bacteria, we used test strains of both groups of bacteria. The antagonistic activity of the studied cultures against pathogenic and opportunistic bacteria was determined by the zone of growth inhibition of indicator strains around the colonies of individual strains of lactobacilli and their consortium (in mm). Priority clinical isolates isolated from birds with intestinal infections were used as indicator cultures: Proteus vulgaris, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Campylobacter jejuni and Campylobacter jejuni ATCC strains 33560. During the study, most bacterial strains of the genus Lactobacillus were highly antagonistic its activity against indicator strains. The most sensitive to the inhibitory effect of lactobacilli were E. coli, Campylobacter jejuni, S. typhimurium and P. vulgaris. The research results showed that the strain L. plantarum ATCC 8014 exhibits a more pronounced antagonistic activity than other strains of lactobacilli.
Keywords: Lactobacilli, Antibiotic resistance, Antibiotics, broilers, Antagonism to Campylobacter jejuni.